2013年2月8日星期五

Sequence assembly with MIRA3, a Swiss knife like tool


Sequence assembly with MIRA3



MIRA is a whole genome shotgun and EST sequence assembler for Sanger, 454, Solexa (Illumina) and PacBio data. It can be seen as a Swiss army knife of sequence assembly developed and used in the past 12 years to get assembly jobs done efficiently - and especially accurately. That is, without actually putting too much manual work into finishing the assembly.

References: 
  • Chevreux et al. (1999) Genome Sequence Assembly Using Trace Signals and Additional Sequence Information Computer Science and Biology: Proceedings of the German Conference on Bioinformatics (GCB) 99, pp. 45-56.
  • Chevreux et al. (2004) Using the miraEST Assembler for Reliable and Automated mRNA Transcript Assembly and SNP Detection in Sequenced ESTs Genome Research 2004. 14:1147-1159.

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