NGS QC Toolkit

NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

Next generation sequencing (NGS) technologies provide ahigh-throughput means to generate large amount ofsequence data. However, quality control (QC) of sequencedata generated from these technologies is extremelyimportant for meaningful downstream analysis. Further,highly efficient and fast processing tools are required tohandle the large volume of datasets. Here, we havedeveloped an application, NGS QC Toolkit, for quality checkand filtering of high-quality data. This toolkit is a standaloneand open source application freely available at http://www.nipgr.res.in/ngsqctoolkit.htm​l. All the tools in the application have been implemented in Perl programming language. The toolkit is comprised of user-friendly tools for QC of sequencing data generated using Roche 454 and Illumina platforms, and additional tools to aid QC (sequence format converter and trimming tools) and analysis (statistics tools). A variety of options have been provided to facilitate the QC at user-defined parameters. The toolkit is expected to be very useful for the QC of NGS data to facilitate better downstream analysis.